Adhesion experiments and analysis of the human pathogen Acanthamoeba castellanii

Published on November 6, 2015

The ubiquitous human pathogenic Acanthamoeba castellanii can cause severe brain or eye infections like Acanthamoeba keratitis (A. keratitis), which occurs mainly due to wrong contact lens use and can lead to complete vision loss. For immune suppressive individuals it can even cause a granulomatous amoebic encephalitis (GAE). Therefore, research on soft polymers comparable to contact lenses are carried out to investigate the pathogenicity of these amoebae and to develop strategies for avoiding infections with these parasites.

Selhuber Group | Christian-Albrechts-University of Kiel, Germany

Prof. Dr. rer. nat. Christine Selhuber-Unkel is head of the group Biocompatible Nanomaterials at the Christian Albrechts-University of Kiel. She and her group study dynamic processes in cells to gain further insight into the interplay of intracellular and extracellular phenomena. A particular focus is in cellular mechanotransduction studies and in transferring such cellular mechanotransduction concepts into novel biomimetic materials.

Sören B. Gutekunst: PhD candidate who is interested in adhesion and force generation of cells by biophysical investigations.

Supattra Paveenkittiporn: Master student who studies cell migration and bioinspired materials.

Katharina Göpfert: Student carrying out polymer synthesis.

Katharina Siemsen: Student studying Acanthamoeba migration inside topographically modified polymers.

Sandra Sindt: Student investigating cell adhesion area and migration with an interference-based microscopy method.

Sources

Reverey, J.F., Fromme, R., Leippe, M., Selhuber-Unkel, C.: In vitro adhesion of Acanthamoeba castellanii to soft contact lenses depends on water content and disinfection procedure Contact Lens Anterio. 2014, 37(4),262–266, DOI: 10.1016/j.clae.2013.11.010

Gutekunst, S.P., Grabosch, C., Kovalev, A., Stanislav N. Gorb, S.N., Selhuber-Unkel, C.: Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii Beilstein J. Nanotechnol. 2014, 44,1393–1398, DOI: 10.3762/bjnano.5.152

[Picture of Acanthapodia] Marciano–Carbal et al.

[Picture of Keratitis (CC BY 2.0)]
J. Lorenzo-Morales et al., published by EDP Sciences, 2015

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