Bioprocessing and protein glycosylation analysis

Published on April 12, 2013

The video shows the NIBRT Training Team in the pilot plant, as they detail the step-by-step manufacturing processes for the production of biotherapeutics.

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The National Institute for Bioprocessing Research and Training (NIBRT) replicates a modern bioprocessing plant with state-of-the-art equipment for the production and analysis of biopharmaceuticals, such as therapeutic monoclonal antibodies (mABs). The video shows the NIBRT Training Team in the pilot plant, as they detail the step-by-step manufacturing processes for the production of biotherapeutics. This is followed by a summary of the analytical technologies used by the NIBRT Glycobiology Research Group to analyse the sugars or “glycans” present on the biopharmaceuticals.

National Institute for Bioprocessing Research and Training, Dublin, Ireland

Professor Pauline M. Rudd heads the Glycobiology Laboratory Research Group at the National Institute for Bioprocessing Research and Training. Before moving her group to University College Dublin in 2006, Professor Rudd was a University Reader in the Glycobiology Institute at Oxford University. Professor Rudd obtained a BSc in Chemistry at the University of London and a PhD in Glycobiology at the Open University, UK. She was a Founding Scientist of Wessex Biochemicals (later Sigma London). She has been a Visiting Research Associate at The Scripps Research Institute, CA, Visiting Professor of Biochemistry at Shanghai Medical University PRC, Visiting Scientist at Ben Gurion University of the Negev, Israel and Erskine Visiting Fellow, Canterbury University, Christchurch, New Zealand. She is a Fellow of the Royal Society of Medicine, London and a Visiting Professor at St. George’s Hospital, London. She is an adjunct professor at North Eastern University, Boston, NUI Galway, University College Dublin and Trinity College Dublin.

Gerard O’Sullivan is a technical trainer in the training department. He specialises in aseptic processing and fill-finish training in NIBRT’s purpose-built pilot plant training facility.

Dr Rebecca Duke is a postdoctoral researcher in the NIBRT Glycobiology Group. Her current research focuses on characterizing the glycans present in human skin.

Sources

The original research article is published in Nature Chemical Biology.

Mariño, K.; Bones, J.; Kattla, J. J.; Rudd, P. M. A systematic approach to protein glycosylation analysis: a path through the maze, Nat Chem Biol. 2010, 6, 713–723. doi:10.1038/nchembio.437.

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