Concept of “Experimental Subset” and input of assay conditions and results data

Published on September 14, 2017

Tutorials on STRENDA DB

Concept of “Experimental Subset” and input of assay conditions and results data

In STRENDA DB an additional term is used, i.e “Experimental Subset” which is explained in details in order to generate the technical understanding required to navigate through the input process. An “Experimental Subset” can be considered a container for those data that define the assay conditions along with those data that define the results from the investigation of the functional activity of an enzyme. Since the enzyme kinetics is strongly dependent of the assay conditions at which the experiment has been carried out, assay conditions and results data are combined. One experiment can contain one or more “Experimental Subsets”. This is very often the case, e.g. when profiles are studied such as pH profiles, substrate specificities or the effect of various modifiers, i.e. inhibitors and activators. After the successful data entry, each experiment is assigned a STRENDA Registry Number (SRN) and a DOI. The conceptual introduction is followed by the entry of example data for the assay conditions and results.

The STRENDA Commission has established standards for data reporting in enzymology research. The aim of these STRENDA Guidelines is to improve the quality of data published in the scientific literature and to enable researchers to compare, evaluate, interpret and reproduce experimental research results published in the literature and databases. STRENDA supports authors by providing STRENDA DB – a web-based database whose data submission form automatically checks the manuscript data prior or during the publication process on compliance with the STRENDA Guidelines.

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