Efficiency of Enzyme Catalysis

Published on November 2, 2017

Interview with Kenneth A. Johnson, University of Texas at Austin, USA

recorded at BEILSTEIN ENZYMOLOGY SYMPOSIUM – Enzymes in Transformation and Signalling
Rüdesheim, Germany, 19 – 21 September 2017

Kenneth Johnson is talking with Carsten Kettner about the appropriate application of terminology in enzymology. Although this terminology is well defined and recommended by, e.g. IUBMB, the major reasons for the confusion caused by the often falsely used terms seems to be laziness of authors and lack of sound training and education. Ken explains the parameters that affect the efficiency of enzyme catalysis: The efficiency of enzymes derives from their flexibility and dynamics. Intramolecular movements within an enzyme result in high specificity in selecting the favourite substrate over the mismatch. He describes this using the example of DNA polymerases where the polymerase has to be fairly discriminatory over very small differences in the structure of the correct base versus the mismatch, and those enzymes have very high specificity at very low error rates. However, mismatch in DNA replication can also have evolutionary impact which can be studied on HIV which can be considered a moving target that alters rapidly recognition sites to hide itself from the immune system until it collapses and the patient gets AIDS.

Please have a look at our other videos with some speakers and their standpoints on Open Science:

Functional Anlysis of Promiscuous Enzymes
Karen N. Allen, Boston University, USA

Directed Evolution of Enzymes
Donald Hilvert, ETH Zurich, Switzerland

Nature is the Master of Design
Roland Wohlgemuth, ESAB, Buchs, Switzerland

Discussion: Data Deposition Increases Credibility
Barbara M. Bakker, Carsten Kettner, Thomas S. Leyh, Johann M. Rohwer, Reinhard Sterner

The Beilstein Enzymology Symposia embrace structural, computational and biological disciplines, and bring researchers (established and younger workers) together to discuss the many and diverse roles of enzymes in biology, and to explore the limits and challenges of holistic studies that attempt to integrate microscopic views of protein function into complex biological behaviour.

This symposium addresses enzymes and other signal‑cascade proteins which capture and transduce these diverse signals into discrete chemical entities that inform and direct cellular behaviour. Molecular-information processing networks require the coordinated interplay of numerous components, and are the focus of systems-biological investigations aimed at understanding, for example, the spread of disease, or identifying targets that can control signal-transduction.

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