Mutasynthetic biotransformations

Published on November 2, 2012

We describe the unprecedented formation of six ansamitocin derivatives that are deoxygenated at C-7 of the ansamitocin core. The ansamitocin core is obtained by fermentation experiments that employ a variety of Actinosynnema pretiosum mutants and mutasynthetic approaches. The video shows in detail how the mutasynthetic experiments are conducted. Additionally, the antiproliferative test of the new ansamitocin derivatives is shown.

Kirschning Group | Leibniz Universität Hannover, Germany

Prof. Dr. Andreas Kirschning has been a full professor at the Institute of Organic Chemistry at the Leibniz University Hannover, Germany since 2000. His group focuses on enabling technologies in organic synthesis including solid-phase-assisted synthesis, microreactors, and novel immobilization strategies for catalysts and methodology development. He is also interested in natural-product synthesis, more specifically structure elucidation, total synthesis and mutasynthesis of biologically active natural products such as antiinfectives and antitumor agents, as well as natural products as tools for drug research and biological target identification.

Dr. Frank Hahn is an assistant professor at the Institute of Organic Chemistry of the Leibniz University Hannover.

Gerrit Jürjens pursues his Ph. D. thesis in the research group of Prof. Andreas Kirschning.

Franziska Sambale pursues her Ph. D. thesis in the group of Prof. Thomas Scheper at the Institute of Technical Chemistry at the Leibniz University Hannover.

Sources

The original research article is published in the Open Access Beilstein Journal of Organic Chemistry and is part of the Thematic Series Biosynthesis and function of secondary metabolites.

Knobloch, T.; Dräger, G.; Collisi, W.; Sasse, F.; Kirschning, A., Unprecedented deoxygenation at C-7 of the ansamitocin core during mutasynthetic biotransformations, Beilstein J. Org. Chem. 2012, 8, 861–869. doi:10.3762/bjoc.8.96

The original research article is published in the Open Access Beilstein Journal of Organic Chemistry and is part of the Thematic Series Biosynthesis and function of secondary metabolites.

Knobloch, T.; Dräger, G.; Collisi, W.; Sasse, F.; Kirschning, A., Unprecedented deoxygenation at C-7 of the ansamitocin core during mutasynthetic biotransformations, Beilstein J. Org. Chem. 2012, 8, 861–869. doi:10.3762/bjoc.8.96

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