Single-molecule fluorescence excitation spectroscopy

Published on June 1, 2012

The video demonstrates how the coupling of quantum emitters, such as single nanoparticles, to an external electromagnetic field can be investigated.

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An experimental configuration is presented, which employs a tunable supercontinuum white-light laser as an excitation source. A grating monochromator facilitates the selection of one wavelength at a time as well as its guidance to a confocal fluorescence microscope. After an isolated emitter is localized by raster scanning, a highly diluted sample prepared on a glass cover slip can be individually addressed and its excitation spectrum can be recorded. This allows for the detection of previously concealed quantum properties. Furthermore, the technique can be applied to sensitively study complex energy-migration processes in biological systems.

Meixner Group | University of Tübingen, Germany

Prof. Dr. Alfred J. Meixner is the director of the Institute of Physical and Theoretical Chemistry at the University of Tübingen. His research interests cover the area of nano-optics and focuses on plasmonics, nearfield microscopy and single-molecule spectroscopy.

Dr. Frank Schleifenbaum is a junior research group leader in the field of biophysical chemistry and affiliated to the Institute of Physical and Theoretical Chemistry and the Center of Plant Molecular Biology at the University of Tübingen. His research interests include single-molecule spectroscopy, live-cell imaging and molecular optical sensors.

Sébastien Peter works as a PhD candidate in the biophysical chemistry group and works on single-molecule spectroscopy and super-resolution microscopy.

Sources

The original research article is published in the Open Access Beilstein Journal of Nanotechnology and is part of the Thematic Series Nanophotonics, nano-optics and nanospectroscopy.

Blum, C.; Schleifenbaum, F.; Stopel, M.; Peter, S.; Sackrow, M; Subramaniam, V.; Meixner, A. J., Room temperature excitation spectroscopy of single quantum dots, Beilstein J. Nanotechnol. 2011, 2, 516–524. doi:10.3762/bjnano.2.56

The original research article is published in the Open Access Beilstein Journal of Nanotechnology and is part of the Thematic Series Nanophotonics, nano-optics and nanospectroscopy.

Blum, C.; Schleifenbaum, F.; Stopel, M.; Peter, S.; Sackrow, M; Subramaniam, V.; Meixner, A. J., Room temperature excitation spectroscopy of single quantum dots, Beilstein J. Nanotechnol. 2011, 2, 516–524. doi:10.3762/bjnano.2.56

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