Structure Elucidation without NMR

Published on July 25, 2014

This video will show how microderivatization of natural extracts containing the target compounds in the ng to μg range can be performed and how the reaction products give additional mass spectral data, helpful for structure elucidation.

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Structure elucidation of organic compounds present in trace amounts is often needed, e. g. in pheromone research. The target compounds occur in small amounts in difficult to separate mixtures, making isolation of pure samples for NMR experiments unsuccessful. Structure elucidation can then only be performed by GC/MS analysis, derivatization of extracts, and synthesis of proposed structures for structural proof.

Schulz Group | Technische Universität Braunschweig, Germany

Prof. Dr. Stefan Schulz is interested in the chemistry of chemical communication with natural compounds and Chemical Ecology. He is professor of Organic Chemistry at the Technische Universität Braunschweig, which he currently chairs. He has published over 160 research papers on chemical communication of various systems ranging from bacteria, insects, arachnids, plants to reptiles and amphibia. Chemical communication is interpreted here in the widest sense, including defense compounds and bacterial antibiotics.

Pardha Saradhi Peram is performing his PhD research in the group of Prof. Schulz and is working on frog pheromones.

PhD student Hilke Bruns is interested in the secondary metabolites of bacteria of the Roseobacter clade.

These important bacteria are also investigated by Lisa Ziesche, who investigates as a PhD student the chemical communication in Roseobacter.

Sources

The methods described can be found in the following publications:

Neumann, A., Patzelt, D., Wagner-Döbel I., Schulz, S.; Identification of new N-acylhomoserine lactone signalling compounds of Dinoroseobacter shibae DFL-12T by overexpression of luxI genes,
ChemBioChem 2013, 25, 2355-2361, doi:10.1002/cbic.201300424

Poth, D., Wollenberg, K., Vences, M., Schulz, S.; Volatile amphibian pheromones: Macrolides of mantellid frogs from Madagascar
Angew. Chem. Int. Ed. 2012, 51, 2187-2190, doi:10.1002/anie.201106592

Stritzke, K., Schulz, S., Boppré, M.; Niaviolides, new macrocyclic sesquiterpenes secreted by males of the African butterfly Amauris niavius,
Eur. J. Org. Chem. 2003, 7, 1337-1342 doi:10.1002/ejoc.200390189

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